Genetic diversity analysis of Bitti (Vitex cofasssus), native species Indonesia based on Inter Simple Sequence Repeat (ISSR) marker

Abstract Bitti ( Vitex cofassus ) is a native species in Indonesia and superior wood South Sulawesi. This type hardwood that sought after exported large enough quantities. A narrow genetic base requires joint efforts to develop plant varieties, so it necessary carry out selection activities obtain diversity of traits. The most advanced method assessing molecularly based approach using the Inter Simple Sequence Repeat (ISSR) marker. Germplasm exploration was carried Pattiroang Village, Kajang District, Bulukumba Regency, Sulawesi Province. total 30 samples were extracted Genomic DNA Mini Kit (plant) with modifications. Ten primers tested obtained six produced bright polymorphic bands, namely UBC 820, 822, 823, 824, 827, 830. highest He value 824 primary PIC 0.48, while lowest values 827 primer 0.15. visualization dendrogram shows two clusters, each consisting diverse number individuals. It indicates relatively moderate kinship between types V. .